Applications: Secondary Screening: Dual NanoCell

Miniaturized FLIPR Assays



2 x Rbt Denso Robot
6 x Mcd MicroDock
1 x Rdr FLIPR Tetra
1 x Pip Acoustic Dispensing Device
1 x Inc HighRes SteriStore D
2 x Car HighRes NanoServe
1 x Plt Automated Plate Washer
1 x Cfg Automated Plate Centrifuge
1 x Dsp Single-Reagent Dispenser
1 x Lid HighRes Lid Valet
1 x Bcr Barcode Reader
1 x Connector


Overview

This system is a dual HighRes NanoCell configured for FLIPR assays. This system would typically be used to run low volume calcium mobilisation assays, for identifying compounds with activity against drug discovery targets, such as GPCRs and ion channels. The capabilities include plate washing and low volume dispensing for removal of cell medium and addition of FLIPR dye, a HighRes SteriStore for incubating cell plates, and an acoustic dispenser for direct addition of compounds in DMSO. The workcell is compatible with all common FLIPR assays, including agonist and antagonist formats.


System Key Advantages

Flexible - Plug in different carts to build the best system for the next experiment. For example, swap out the FLIPR for a multimode reader

Economical - Minimise duplicate equipment purchases - undock the FLIPR for method writing and maintenance, then plug it straight back into the system for automated use

Friendly - Benefit from the intuitive interface of Cellario, HighRes’ scheduling software, making the system easy to use for non-frequent users

Efficient - To get the most out of the system, use Cellario to schedule additional protocols (e.g. cell plating) that can run during overnight periods


More Information

HighRes has co-authored a technical note with Molecular Devices on the advantages of using a NanoCell for automated, miniaturized FLIPR screening.

Download this Technical Note.