Cell-Based Screening

Performing Cell based assays can often require labor intensive, highly complex protocols that must be executed efficiently and accurately on large numbers of samples. Given both the complexity of the procedures and the desired sample throughput, the use of laboratory automation has been a key stepping stone for many laboratories working in this area. Here are examples of some of the systems HighRes has created for Cell based assays:

This system is a BSL2 enclosed screening system that is designed to handle a variety of cell based assays for a MTS/HTS and Lead Optimization team. This NanoCell design densely packages all of the equipment required to run a vast array of cell based assays. The lab had limited floor space and a need for a large number of assays to be run in a clean environment. The design introduced MicroDocks on the key flex points of the SteriStore incubator and reader position. This choice allows the system to morph functions by docking in different readers and therefore increasing the range of assays that can be performed on the same system. The customer also chose to implement a smaller ambient storage and tewo dock system in their lab that essentially serves as a reader feeder to automate the readers that are not in use on the larger platform. Similarly, docking the incubator allows cells to be grown offline and then docked onto the system. There are a variety of additional assay peripherals that are placed onto fixed positions on the system including dispensers, a centrifuges, a pipettor and a washer.

  • Cell based screening system
  • Cell based screening system
  • Cell based screening system
  • Cell based screening system

This is a High Content Imaging (HCI) screening system that enables both live and fixed cell imaging. The system must was designed to easily adapt to differing assay protocols, while maintaining a relatively small footprint. It is easy to operate, has accessible hardware and a dynamic scheduling software with adaptable error recovery as well as positive plate positioning, the ability to run multiple schedules in parallel, and rapid exchange of peripherals. The system is able to process barcoded and lidded cell/assay plates, barcoded and sealed compound plates, acoustic compound transfer, bulk reagent dispensing , plate washing, centrifugation, sealing/unsealing, lidding and delidding.

  • Cell base screening system
  • Cell base screening system

The client’s primary goal was to perform high content imaging assays separately from their main screening system. They also had a need to repurpose the equipment on this HCI system on their main screening system. We developed this CoLAB Flex based system to perform their fix-stain-imaging assays (2 day continuous assay). As their assay incorporates paraformaldehyde (PFA) to fix their cells, each tier of the CoLAB Flex cart is partially enclosed and contains an HVAC attachment port to protect the scientists from PFA vapors. Each device on this system can be undocked, moved to main screening system and docked into place. This allows the client repurpose the equipment when needed

  • Cell base screening system
  • Cell base screening system

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