Designed as both a cell culture and cell assay solution, the system is designed to perform cell seeding, cell dosing, and up to 3 different assays over 3 days. The protocol requires the liquid handler to perform selective pipetting serial dilutions during the dosing process while also adding both positive and negative controls in the cell plate. The system incorporates the HighRes MicroDock and MicroCart technology allowing the client to exchange cell lines by exchanging incubators actively on the system. The client also uses the MicroCart technology to reconfigure the system to perform RNAi screening.
Designed to perform every step in a cell culture workflow (cell seeding, media exchange, media transfer) in preparation for cell screening. The system is designed to hold up to 600 cell plates consisting of 3 different cell lines. Cell lines are cultured over a two week period with up to four feedings depending on the cell line. The system interleaves the cell lines for maximum throughput. At the end of the culture period, media is then transferred into 38 – 50 x 384 well plates where those plates are transferred to a cell screening system. Following the screens, hit data is imported back into Cellario and a cherry pick workflow is executed from the original 96 well cell plates into other plate formats (24 or 48 well) for culture expansion